PRIMERS
Overview
In order to obtain
barcode sequences from such a broad spectrum of animal
taxa, researchers require an arsenal of primer sets.
Some primers will amplify COI across a wide range of
organisms, while others may be designed to target specific
taxonomic groups. A brief summary of the primers used
to barcode animal life is given below:
Folmer Primer Set
The primer set LCO1490
and HCO2198 amplify a 658 bp fragment of the COI gene
in a wide range of invertebrate taxa (Folmer et al.
1994).
LCO1490: 5'-GGTCAACAAATCATAAAGATATTGG-3'
HCO2198: 5'-TAAACTTCAGGGTGACCAAAAAATCA-3'
Reference
Folmer, O., Black, M., Hoeh,
W., Lutz, R. & Vrijenhoek, R. 1994. DNA primers
for amplification of mitochondrial cytochrome c oxidase
subunit I from diverse metazoan invertebrates. Mol.
Mar. Biol. Biotechnol. 3, 294-297.
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