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PAST WORK
Information Content
The mitochondrial genome includes just 13 protein-coding genes that might serve as the core of a DNA-based identification system. The length of each gene varies little across the animal kingdom, but there is more than a 10-fold difference among genes. This length variation acts to exclude most genes from consideration, as a minimum length of 900 bp is required to make best use of existing technologies. Current sequencers may only routinely recover 700 BP, but internal PCR primers must be used to generate the DNA for sequencing to avoid complications which would arise from shifts in gene order if external primers were used. In fact, only four mitochondrial genes have a minimum length in excess of this 900 BP threshold - COI, Cyt b ND4, and ND5.

Minimum reported lengths of the 13 mitochondrial
protein-coding genes in two phyla.
GENE
ARTHROPODA
CHORDATA
 ND5
1626
1779
 COI
1530
1545
 ND4
1119
1338
 Cyt b
1098
1116
 ND1
894
954
 ND2
891
1029
 COIII
774
780
 COII
660
684
 ATP6
657
678
 ND6
426
501
 ND3
336
445
 ND42
258
288
ATP8I
156
159