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The mitochondrial
genome includes just 13 protein-coding genes that might
serve as the core of a DNA-based identification system.
The length of each gene varies little across the animal
kingdom, but there is more than a 10-fold difference
among genes. This length variation acts to exclude most
genes from consideration, as a minimum length of 900
bp is required to make best use of existing technologies.
Current sequencers may only routinely recover 700 BP,
but internal PCR primers must be used to generate the
DNA for sequencing to avoid complications which would
arise from shifts in gene order if external primers
were used. In fact, only four mitochondrial genes have
a minimum length in excess of this 900 BP threshold
- COI, Cyt b ND4, and ND5.
Minimum
reported lengths of the 13 mitochondrial
protein-coding genes in two phyla.
| GENE |
ARTHROPODA |
CHORDATA |
ND5
|
1626 |
1779 |
| COI |
1530 |
1545 |
| ND4 |
1119 |
1338 |
| Cyt
b |
1098 |
1116 |
| ND1 |
894 |
954 |
| ND2 |
891 |
1029 |
| COIII |
774 |
780 |
| COII |
660 |
684 |
| ATP6 |
657 |
678 |
| ND6 |
426 |
501 |
| ND3 |
336 |
445 |
| ND42 |
258 |
288 |
| ATP8I |
156 |
159 |
|